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HPLC法同时测定牛黄清感胶囊中8种有效成分的含量
庄岩1, 遆铁军2, 霍金海1, 朱立刚2, 赵雪莹3, 周有财2, 王伟明1
1.黑龙江省中医药科学院 中药研究所, 哈尔滨 150036;2.黑龙江澳利达奈德制药有限公司, 哈尔滨 150001;3.黑龙江中医药大学, 哈尔滨 150040
摘要:
[目的] 建立牛黄清感胶囊HPLC含量测定,用于该制剂的质量控制。[方法] 运用Waters Alliance 2695高效液相色谱仪,采用C18色谱柱(4.6 mm×250 mm,5 μm),流动相为乙腈-0.5%磷酸溶液,梯度洗脱,柱温40℃,流速为1.0 mL/min,检测波长为350 nm。[结果] 建立同时测定牛黄清感胶囊8个成分隐绿原酸,绿原酸,异绿原酸C,连翘酯苷A,黄芩苷,黄芩素,汉黄芩苷和汉黄芩素的方法。[结论] 该方法稳定、准确、重现性好,专属性强,可用于牛黄清感胶囊的质量控制。
关键词:  牛黄清感胶囊  高效液相色谱  隐绿原酸  黄芩素  连翘酯苷A
DOI:10.11656/j.issn.1673-9043.2019.01.16
分类号:R284.1
基金项目:
Simultaneous Determination of Eight Active Ingredients in Niuhuang Qinggan Capsules by HPLC Method
ZHUANG Yan1, DI Tiejun2, HUO Jinhai1, ZHU Ligang2, ZHAO Xueying3, ZHOU Youcai2, WANG Weiming1
1.Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China;2.Heilongjiang Aolidaned Pharmaceutical Co., Ltd, Harbin 150031, China;3.Heilongjiang University Of Chinese Medicine, Harbin 150040, China
Abstract:
[Objective] To establish HPLC determination of Niuhuang Qinggan capsule for quality control of the preparation.[Methods] Waters Alliance 2695 HPLC is used on a C18 column (4.6 mm×250 mm,5 μm). The mobile phase was acetonitrile-0.5% phosphoric acid solution. Elution with gradient,column temperature is 40°,the flow rate is 1.0 mL/min,detection wavelength is 350 nm.[Reslts] A method was established for the simultaneous determination of cryptochlorogenic acid,chlorogenic acid,isochlorogenic acid C,forsythiaside A,baicalin,baicalein,wogonin and wogonin in 8 components of Niuhuang Qinggan Capsules.[Conclusion] This method is stable,accurate,reproducible,and highly specific. It can be used for the quality control of Niu Huang Qinggan capsules.
Key words:  Niuhuang Qinggan capsule  HPLC  cryptochlorogenic acid  baicalein  forsythiaside A
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